isolation and characterization of the progenitor cells from the blastema tissue formed at experimentally-created rabbit ear hole

نویسندگان

mohamadreza baghaban eslaminejad 1department of stem cells and developmental biology, cell science research centre, royan institute for stem cell biology and technology, acecr, tehran, iran

sima bordbar 1department of stem cells and developmental biology, cell science research centre, royan institute for stem cell biology and technology, acecr, tehran, iran

چکیده

objective(s): throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. the objective of the present study is to isolate, culture expand, and characterize blastema progenitor cells in terms of their in vitro differentiation capacity.   materials and methods: five new zealand white male rabbits were used in the present study. using a punching apparatus, a 4-mm hole was created in the animal ears. following 4 days, the blastema ring which was created in the periphery of primary hole in the ears was removed and cultivated. the cells migrated from the blastema were expanded through 3 successive subcultures and characterized in terms of their potential differentiation, growth characteristics, and culture requirements. results: the primary cultures tended to be morphologically heterogeneous having spindly-shaped fibroblast-like cells as well as flattened cells. fibroblast-like cells survived and dominated the cultures. these cells tended to have the osteogenic, chondrogenic, and adipogenic differentiation potentials. they were highly colonogenic and maximum proliferation was achieved when the cells were plated at density of 100 cells/cm2 in a medium which contained 10% fetal bovine serum (fbs). conclusion: taken together, blastema tissue-derived stem cells from rabbit ear are of mesenchymal stem cell-like population. studies similar to this will assist scientist better understanding the nature of blastema tissue formed at rabbit ear to regenerate the wound.

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منابع مشابه

Isolation and Characterization of the Progenitor Cells From the Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole

  Objective(s): Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, culture expa...

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Isolation and Characterization of the Progenitor Cells From the Blastema Tissue Formed at Experimentally-Created Rabbit Ear Hole

UNLABELLED Objective(s) : Throughout evolution, mammalians have increasingly lost their ability to regenerate structures however rabbits are exceptional since they develop a blastema in their ear wound for regeneration purposes. Blastema consists of a group of undifferentiated cells capable of dividing and differentiating into the ear tissue. The objective of the present study is to isolate, cu...

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Blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

Rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. The purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (MSCs). Five New Zealand white male rabbits were used in the present study. A 2-mm hole was created in the a...

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blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. the purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (mscs). five new zealand white male rabbits were used in the present study. a 2-mm hole was created in the a...

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Blastema from rabbit ear contains progenitor cells comparable to marrow derived mesenchymal stem cells

Rabbits have the capacity to regenerate holes in their ears by forming a blastema, a tissue that is made up of a group of undifferentiated cells. The purpose of the present study was to isolate and characterize blastema progenitor cells and compare them with marrow mesenchymal stem cells (MSCs). Five New Zealand white male rabbits were used in the present study. A 2-mm hole was created in the a...

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عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۶، شماره ۲، صفحات ۱۰۹-۱۱۵

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